Targeted sequencing library preparation by genomic DNA circularization
نویسندگان
چکیده
منابع مشابه
Targeted sequencing library preparation by genomic DNA circularization
BACKGROUND For next generation DNA sequencing, we have developed a rapid and simple approach for preparing DNA libraries of targeted DNA content. Current protocols for preparing DNA for next-generation targeted sequencing are labor-intensive, require large amounts of starting material, and are prone to artifacts that result from necessary PCR amplification of sequencing libraries. Typically, sa...
متن کاملOptimization of genomic DNA shearing by sonication for next-generation sequencing library preparation
* Author for correspondence: Dr. Maha Abdullah, Immunology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia. Tel.: 603-89472375, Fax: 603-89412787, Email: [email protected]. Abstract. Next-generation sequencing (NGS) technologies, capable of sequencing genomic DNA and RNA at high throughput with unprecedented speed, hav...
متن کاملIllumina Library Preparation for Sequencing the GC-Rich Fraction of Heterogeneous Genomic DNA
Standard Illumina libraries are biased toward sequences of intermediate GC-content. This results in an underrepresentation of GC-rich regions in sequencing projects of genomes with heterogeneous base composition, such as mammals and birds. We developed a simple, cost-effective protocol to enrich sheared genomic DNA in its GC-rich fraction by subtracting AT-rich DNA. This was achieved by heating...
متن کاملA Microfluidic DNA Library Preparation Platform for Next-Generation Sequencing
Next-generation sequencing (NGS) is emerging as a powerful tool for elucidating genetic information for a wide range of applications. Unfortunately, the surging popularity of NGS has not yet been accompanied by an improvement in automated techniques for preparing formatted sequencing libraries. To address this challenge, we have developed a prototype microfluidic system for preparing sequencer-...
متن کاملSemi-Automated Library Preparation for High-Throughput DNA Sequencing Platforms
Next-generation sequencing platforms are powerful technologies, providing gigabases of genetic information in a single run. An important prerequisite for high-throughput DNA sequencing is the development of robust and cost-effective preprocessing protocols for DNA sample library construction. Here we report the development of a semi-automated sample preparation protocol to produce adaptor-ligat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2011
ISSN: 1472-6750
DOI: 10.1186/1472-6750-11-122